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Nextera™ Technology for Next-Generation Sequencing Library Preparation
EPICENTRE's revolutionary Nextera™ technology* uses in vitro transposition to
prepare sequencer-ready libraries from genomic DNA for multiple sequencing platforms.
The technology simultaneously fragments and tags DNA, in a single-tube reaction.
· Use nanogram amounts of starting DNA.
· Prepare sequencer-ready libraries in less than 2 hours.
· Incorporate platform-specific tags and optional barcodes.
· Validated on Roche 454™ GS FLX and GS FLX Titanium™, and Illumina® Solexa® GAI, GAII.
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Library Preparation Comparison |
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Processing Step |
Standard (~µg) |
Nextera™ (50 ng) |
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Fragmentation |
✓ (15-30 min) |
Add Nextera™ Enzyme Mix
(5 min) |
|
Collection |
✓ (15 min) |
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Concentration |
✓ (15 min) |
|
Size Selection |
✓ (60 min) |
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End-Repair |
✓ (60 min) |
|
Clean-Up |
✓ (15 min) |
|
A-Tailing |
+/- (30 min) |
|
Adaptor Ligation |
✓ (60 min) |
|
Clean-Up |
✓ (15 min) |
✓ (15 min) |
|
Library Enrichment |
Variable (~60 min) |
PCR (~60 min) |
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Total Time: |
~6 Hours |
<2 Hours | | 
