Mononuclear cells are prepared by centrifugation in a density cell separation medium (Ficoll-Paque?, Amersham Pharmacia) from bone marrow. Cells are available fresh or cryopreserved. When shipped fresh, cells are suspended in HBSS with 5 mM EDTA and 0.5% BSA. Orders for fresh cells should be scheduled 1-2 weeks in advance. |